Ind Out If The Rate Of Glucose Uptake By Yeast Cells Varies With Glucose Concentration.
Glucose is absorbed across the cell surface membrane(plasma membrane) of most cells. A convinient way to investigate this is to use a solution of glucose and a suspension of yeast cells. The amount of glucose taken up from the glucose solution by yeast cells in a fixed length of time can be measured. At the end of the fixed length of time, furthur uptake of glucose is prevented by transferring the yeast suspension to a boiling water bath to kill the yeast cells. If the suspension is then left to stand, the yeast cells form a sediment at the bottom of the tube. The concentration of glucose that remains in the fluid (supernatant) above the sediment can then be determined. As the original concentration of glucose is known, the rate of uptake can be determined.
The glucose concentration of a solution may be determined using a quantitative Benedicts Test in which the solution is boiled with excess Benedicts reagent. The mixture is filtered through dried and weighed filter paper and the mass of the precipitate determined.
There is a direct relationship betwen the concentration of glucose and the mass of the precipitate.
Material and resources:
+10% glucose solution
Give full details of the plan including:
+materials to be used
+detailed method which includes the procedures that you would adopt to ensure that the results obtained would be as precise and reliable as possible.
Indicate how you would present and anaylse your data to draw conclusions.
Please use the descriptors and criteria for SKILL P OF THE A2 BIOLOGY SPECIFICATION THAT IS ATTACHED.
QUALITY OF WRITTEN COMMUNICATION WILL BE ASSESSED.