NA binding assay (Principles of the electro-mobility shift assay and detection of in vitro DNA binding)

In discussion you need to talk about the trouble shooting I had on my result for number 8,9 and 10. (attached in the file of DNA binding).Maybe it was because of the high concentration or maybe the membrane has touched or anything else that you would things might happen and how can improve this in future and also give references to support what you writing. Scientific reason please. You can see the method of this practical in the file that I have attached for you. Please explain each point in the result in discussion and give conclusion at the end please. written as scientific and please make sure the grammer and spelling is correct(so important for me). the aim/abstract of the practical report has written for you in the same file as result (DNA binding file).