NA methylation effects in Friedreich ataxia (FRDA) ; Review of DNA methylation mechanisms and effects (Genetics)
I have attached the project title, here is a summary
DNA methylation effects in Friedreich ataxia (FRDA)
There is good evidence that DNA methylation at promoter sequences can inhibit gene expression. However, the DNA methylation status of the FRDA promoter, and how this may interact with GAA repeat expansion mutations, is unknown. This project will analyse DNA methylation at the FRDA promoter in FRDA transgenic mouse tissues that either do or do not contain GAA repeat expansion mutations.
Literature review, 5000 words plus or minus 250:Review of DNA methylation mechanisms and effects.
Practical component, I will be working on at the same time, but the literature review will have to be tied in with it. It involves: (DNA preparation, bisulphite treatment, methylation sensitive PCR (MSP))
I am including a copy of the guidelines which need to be followed carefully for a 1st classs degree to be possible. So please pay close attention to it, and read it carefully.
Other than that, if you have any further queries please feel free to contact me. I am familiar with what the contents should be, and if I can not help you, I can qury it from my professor and give you a better idea of what is required here.
It is essential for all the referncing to be in the Harvard Referencing format. A good starting place for general background on the FRDA gene, recommended by my professor is the OMIM website; dispomim.cgi?id606829
A lot of useful references can be found from here.
Another source I would like you to look through is the last relevant paper published by my professor on the subject, Rescue of Friedreichs Ataxia knockout mouse by human YAC transgenesis , 2001 which i have attached for your easy reference.
Please, I beg of you to be careful with plagarism. My professor knows all the relevant articles far too well. I will be kicked out of university. (:-(