NA repair mechanisms role in survival to nucleoside analogues treatment in S. pombe .

Identification and analysis of DNA repair mechanisms that contribute to resistance against nucleoside analogues.

– DNA repair mechanisms role in survival to nucleoside analogues treatment in S. pombe .

Solid Media for plates:
EMM 109.2 g
Glutamic acid 15 g
Granulated Agar 10 g
all of them in 4 L dH2O

Liquid media for cultures:
5 ml of EMM+Glut in Falcon tubes with scratch of each culture and incubate at 30C

Drug:
dFdC (Gemcitabine )

Stock solution in H2O 10mM

Strains and result:

1/ WT: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 URA4aim
2/ Swi 10: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 swi10::KanMX URA4aim
3/ exo1: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 exo1::URA
4/ rhp14: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 rhp14:: KanMX URA4aim
5/ rad50: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 rad50:: KanMX URA4aim
6/ rad32D65N: h+ ura4-D18::adhdmdNK-NAT-adhhENT1 rad32D65N URA4aim