O lung epithelia in COPD undergo transition to behave more like fibroblasts ? An immunohistochemical analysis of COPD tissue
Can you please write the result and the discussion part of my dissertation
I will provide you with the sources ( please use the sources which Im providing)and results obtained from the experiment
Added on 02.03.2015 17:06
please write this as an extended version of research paper it should be very scientific
can you also update me regularly with the progress so I can show it to my supervisor
any question please Do ask
About the project :
Chronic Obstructive Pulmonary Disease (COPD) is related to cigarette smoking and is defined by poorly reversible lung function decline. A key pathology of COPD is airway wall thickening, a consequence of fibroblast proliferation and activation (fibrosis). One possible response of epithelial cells to smoke is to undergo a transformation into a fibroblastic type of cell. This process is called epithelial to mesenchymal transition (EMT). It is not known if these fibroblast like cells posess full fibrolast like activity and a key area of research is to better understand their role in COPD pathology.
The student will use immunohistochemical (IHC) techniques to find evidence of EMT and fibrolast activity using available antibodies, in paraffin embedded lung tissue resections of patients with respiratory disease. This will be correlated against morphological appearance of lung resections and patient demographics.
References: Kasai H, Allen JT, Mason RM et al. (2005) TGF-b1 induces human alveolar epithelial to mesenchymal cell transition (EMT). Respiratory Research 6:56. Expression of profibrotic mediators in small airways versus parenchyma after cigarette smoke exposure. Churg A, et al. (2009) Am J Respir Cell Mol Biol. 40(3):268-76. Hogg JC, Chu F, Utokaparch S, et al. (2004) The nature of small airway obstruction in chronic obstructive pulmonary disease. N Engl J Med.24:2645-2653.