Ritically evaluate the use of indirect immunocytochemical techniques in diagnostic pathology

the essay sud include the following…

Introduction

Main body of answer
Definitions, principles and development of IHC
Distinguish between direct and indirect methods/criterion for use
Types of indirect IHC methods available: e.g.
Fluorescence /enzyme based
PAP/APAAP/ABC
Use of monoclonal/polyclonal antibodies
Tissue preparations prior to IHC, e.g.
Fixation
Paraffin or cryostat
Antigen retrieval:
Why is it needed
Methods used: e.g. enzymatic/pressure cooker/microwave
Types of labels/antibodies used/specific examples
Advantages/disadvantages of indirect IHC
Specificity and sensitivity
Application/use in diagnostic pathology
Immuo gold techniques

Concluding remarks: